Sds Plus
SDS-PAGE and OMPs plus LPS samples?
I know that this is probably a bit too complex for yahoo answers (no insult intended) but I’m damn stuck and my lecturers are being useless. I done a lab where I used bacterial outer membrane proteins (OMPs) and lipopolysaccharide (LPS) and ran them on an SDS-PAGE but I have no idea how to interpret the results! I have the print out of the bands but I don’t know what it means about the proteins and how to relate that to the bacterial cell function. I know this is useless as you can’t see my results but I just need someone to tell me what things, other that molecular weight, can the SDS-PAGE show?
you should have quite a smear with whole bacteria outer membrane proteins on a gel. LPS, however, is not a protein and would probably do some weird things on a gel if it would run at all. it would also depend how you analyzed the gel — coomassie stain probably wouldn’t stain LPS.
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